Changes

=== '''the Dendronephthya gigantea 게놈 어셈블리와 다른   자포동물(Cnidarians) 비교통계''' ===

 

우리는 두 가지 다른 방법을 사용하여  D. gigantea에서 29,000개에 가까운 단백질 코딩 유전자(protein-coding genes)를 발견했다(보완표 1, 온라인 보충자료 참조). 1차 접근방식과 2차 접근방식은 각각 D. gigantea게놈의 단백질 코딩 유전자(protein-coding genes)를 2만8879개, 2만8937개로 예측했다. 비교 가능한 높은 품질을 보인 BUSCO (version 3.0.2) (Simão et al. 2015; Waterhouse et al. 2018)를 사용하여 두 유전자 세트를 비교하여 예측 유전자 세트에 대한 신뢰도를 높였다. 첫 번째 방법으로 얻은 유전자 세트는 두 번째 방법(93.35%)보다 약간 높은 품질(93.97% 완성 BUSCO 유전자)을 보였다(부록표 2, 보충재료 온라인) D. Giganta 유전자 세트는 cnidians 중에서 품질이 높았으며 complete BUSCO ortholog benchmark genes의 ~94%를 차지했다(부록 그림 2, Supplementary Material 온라인).  D. gigantea 유전자 모델의 품질을 6명의 cnidarians (Aiptasia pallida, Acropora digitifera, Hydra magnipapillata, Nematostella vectensis, Ovidicella faveolata, Stylophora pistillata)와 비교했다. D. Giganta 유전자 모델은  single copy BUSCO genes을 ~87% 완성했다(부록 그림 2, Supplementary Material 온라인). 또한 cnidarian(부록 그림 2, Supplementary Material online) 중 single copy 및 duplicated genes를 모두 포함하는 complete BUSCO genes (~94%)가 두 번째로 높았다. D. gigantea 게놈의 거의 12%가 반복 요소(repeat elements)로 이루어져 있다. D. giganta 게놈의 11.97%를 차지하는 것을 발견했으며, tandem repeatment와 long terminal repeat 요소(LTR)가 각각 게놈의 7.24%와 2.25%를 나타낸다. (부록표 3, 보충 자료 온라인).

'''무화과. 1.'''

—Dendronephthya gigantea 및 기타 종의 생리 학적 관계 및 Hox 유전자 클러스터. (A) 나무는 15 종 중 발산 시간이있는 계통 발생을 보여준다. 각 분기의 숫자는 예상 발산 시간 (MYA)을 나타낸다. (B) 녹색 점선 박스는 Hox 유전자 클러스터 (HoxA, HoxB, HoxC, HoxDa, HoxDb, HoxE 및 HoxF)를 나타내고, 노란색 점선 박스는 EGF 유전자 클러스터 (Evex 및 Gbx)를 나타내며, 청색 점선 박스를 나타낸다 는 ParaHox 유전자 클러스터 (CDX 및 GSX)를 나타냅니다. 박스 수는 게놈에서 각 유전자 카피 수를 나타낸다.

또한 연한 산호와 돌이 많은 산호 사이의 Hox (Homeobox) 유전자의 차이점을 조사했다. Hox 유전자는 발달 동안 다양한 역할을 수행하는 전사 인자를 암호화한다 (Akam 1995). 그들은 신체 계획을 정의하는 것으로 가장 잘 알려져 있다 (Akam 1995). 우리는 3 개의 돌이 많은 산호가 유사하고 친숙한 Hox 유전자 클러스터 패턴을 가지고 있음을 발견했다 (Ying et al. 2018) (그림 1B). 그러나 Hox 유전자의 Antp 수퍼 클래스 (Patel and Prince 2000)의 구성원 인 Evx는 D. gigantea (그림 1B)에는 실험적으로 검증되어야 할 발견이 없다. 

== '''인용문헌''' ==

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